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Friday, February 21, 2020

Paso doble

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You may not be able to see or even hear my next guests because theyre all women over the age of 60 and, according to them, that makes them invisible, which is a pity because theyve got things they really want you to hear. Nevertheless, please make them welcome. Ladies and gentlemen, the Older Womens Network Theatre Group.


(Woman plays lively piano melody) Do my essay on paso doble CHEAP !


(Women sing)


Older Women, thats our name


Fighting for rights is our game


We stand up here with our claim


Being older is no shame


Were not seeking for great fame


But were proud to say our name


Just want to be there in the frame


Cause Older Women, thats our name


Grey hair and wrinkles are OK


Just the thing for cabaret


Being curvaceous, thats our way


You cant say that were pass


Social justice is our aim


Were standing firm to exclaim


Wont stop and wont be tame


Cause Older Women, thats our name.


(All chant)


Older Women, Older Women, oi oi oi.


Wont stop and wont be tame


Cause Older Women, thats our name.


APPLAUSE


Andrew Denton Lets meet now two of the Older Womens Network. With me, Louise Anike and Josie Jackson. Come on over. Make them welcome, please.


Josie Jackson Hooray!


APPLAUSE


Andrew Denton Lets meet now two of the Older Womens Network. With me, Louise Anike and Josie Jackson. Come on over. Make them welcome, please.


Josie Jackson Hooray!


APPLAUSE


Andrew Denton No, dont be sorry. Were all coming to it. I…I can assure you. So, what are the sort of things… We heard that introductory song. What sort of things do you sing about?


Louise Anike Oh, dearie me. Everything.


Josie Jackson Public transport.


Louise Anike Transport, Telstra selling off of Telstra.


Josie Jackson Banks.


Louise Anike Banks.


Andrew Denton No, dont be sorry. Were all coming to it. I…I can assure you. So, what are the sort of things… We heard that introductory song. What sort of things do you sing about?


Louise Anike Oh, dearie me. Everything.


Josie Jackson Public transport.


Louise Anike Transport, Telstra selling off of Telstra.


Josie Jackson Banks.


Louise Anike Banks.


you. Why is that?


Louise Anike (Sighs)


Josie Jackson You…when you get on a bus, you usually… the steps are too high for a lot of older people. They get arthritis and, you know, all the other crap that goes with getting old. You get on the bus and then you… before you can get your bum on a seat, the driver starts off, because his job is spoiled by the passengers he picks up. If he didnt have passengers, hed be OK.


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Thursday, February 20, 2020

Hiv

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PRACTICAL 5


AMYLASE ACTIVITY IN GERMINATING BARLEY


INTRODUCTION


Cheap University Papers on hiv


Thousand of chemical reactions occur each instant throughout the body; this coordinated process of chemical change is named metabolism.


Metabolism includes the synthesis and breakdown of organic molecules required for cell structure and function and the release of chemical energy used for cell functions.(Vander, Sherman & Luciano, 001)


The bodys organic molecule undergo continuous transformation as some molecules are broken down while others of the same type are being synthesized.


Germination is the resumption of growth of the embryo plant inside the seed.


Seeds remain dormant or inactive until conditions are right for germination. All seeds need water, oxygen, and proper temperature in order to germinate, some seeds require proper light also. Some germinate better in full light while others require darkness to germinate.


When a seed is exposed to the proper conditions, water and oxygen are taken in through the seed coat. The embryos cells start to enlarge. Then the seed coat breaks open ( and also triggers metabolic changes in the embryo) and a radicle, the embryonic root emerges first, followed by the shoot or plumule that contains the leaves and stem.


In a germinating barley seed, food reserves stored in the endosperm, (as glucose polymer starch) and during seed germination, the starch is hydrolysed and mobilized by enzyme amylase into maltose.


This enzyme hydrolyses the a (1-4) glycosidic bonds between pairs of glucose units to release the disaccharide maltose. Maltose is then hydrolysed to single glucose molecules by a second enzyme called glycosidase. Glucose can enter the glycolytic pathway where it is utilized for producing ATP and carbon molecules for biosynthesis.( Campbell & Reece, 00), when the starch reserves are exhausted, the seedling has usually reached a stage where energy and carbon requirements can be met by photosynthesis.


Photosynthesis is the process by which plants make food, is the process by which sugars and more complex compounds are made from carbon dioxide and water in cells containing chloroplasts. The chemical energy is produced from solar energy in the presence of the pigment chlorophyll.


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The aim of this exercise is to determine the comparative amylase activity in the dormant barley seeds, germinating barley seeds and barley seedlings by measuring their rates of starch hydrolysis, and to interpret this results in terms of the metabolic role of amylase during plant development.


MATERIALS AND METHODS


For the preparation of amylase extract from germinating barley, 10 grains were crushed with mortar and pestle to a fine paste, and 10ml of buffer was added, to extract the amylase into solution. The extract was filtered into a 100ml beaker, and the volume was recorded.


A five-fold dilution of the filtered extract was made by adding 5 ml to the measuring cylinder and buffer was added to make the total volume 5ml.


The control extract was prepared by adding 5ml of the diluted filtered extract to a test tube and place it in the boiling water bath for 10minutes, after that period of time it was removed from the boiling water bath and leave it to cool at room temperature.


And for the 10 dormant barley seeds and 10 whole barley seedlings the same method was used, including the use of boiled controls.


Determination of amylase activity in germinating barley by measuring the rate of starch hydrolysis.


The rate of starch hydrolysis can be determined by making up a reaction mixture containing starch in a buffer of appropriate pH. Amylase extract is added, and the samples are tested with starch indicator (iodine) it gives a blue- black colour when bound to starch, and the samples are tested at constant time intervals of 1 minute


RESULTS


Table 1 represents a description of the amylase activity of developmental stages of barley, and time taken to reach achromic point.


NUMBER OF GRAINSTYPES OF BARLEY SEEDSFILTRATE VOLUMETIME


AMYLASE ACTIVITY LEVEL


10GERMINATING BARLEY8.5ML MINUTES Quite High


10DORMANT BARLEY8MLNO COLOUR CHANGE No reaction


10WHOLE BARLEY SD.ML1 MIN.Quite high


Among these developmental stages of barley, the amylase activity was highest in the germinating barley, followed by whole barley seedling, with dormant barley seeds showing not activity at all ( Table 1).


Calculation of amylase activity in the germinating barley


Concentration of the starch= 0.5% = 500mg/100ml = 5mg/ml


i) So, for 1ml of solution = 5mg/ml x 1ml = 5mg of starch


time taken to reach the achromic point for the germinating barley = minutes


ii)average amount of starch hydrolysed/min to reach the achromic point =5mg = 0.55mg/min


min


iii) give ten grains were used to make the extract, number of grains per ml


= number of grains =10grains = 1. grain/ml


number of ml extract8.5ml


= 1. grain/ml = 0. grains/ml x 1ml = 0. grains


5ml


So, mg starch hydrolysed/min/barley grain


= number of mg/ minute hydrolysed= 0.55mg/min = .75 mg/min/grains


number of grains 0.grains


Amylase activity = .75 mg/minute/grain for germinating barley.


For the amylase activity for the dormant and whole seedling refer to the table


Table represents amylase activity for different types of seeds


TYPES OF BARLEY SEEDSAmylase activity ( mg/min/grain)


Germinating seed.75


Dormant seed0


Whole seedling


In this table we are just reinforcing what was stated in table 1- but here we can key in number by saying -


Amylase activity was highest in the germinating experimental treatment, which had an activity of .75 mg/min/grain followed by the whole seedling experimental which had an activity of mg/min/grain and followed by the dormant seeds which did not show any amylase activity at all.


Table represents reaction of different mixtures with Benedicts reagent to determine maltose


Reaction mixtureresults


4 a) reaction mixture from (d)Red-yellow, greenish colour


4 b) controlBright blue color


Table indicates that cuprous oxide precipitate was present in the reaction tube containing amylase extract (from part d), as indicated by its resulting red-yellow, greenish colour.


Discussion


In terms of the metabolic rate of amylase during barley plant development, in the germinating barley seed , a lot of energy (ATP) is needed for cellular respiration, and for the seedling to germinate, so a lot of amylase is required in order to breakdown starch.


The amylase is able to breakdown starch into maltose, but unable to breakdown the cellulose in the cell wall into smaller molecules, despite both being polymers of glucose because as amylase is an enzyme it is very specific as to type of bonds it breaks down


In the dormant seed very little/ no amylase is produced, for the reason that as the seed is not growing or developing, very little energy is needed in order to respire, therefore minimal amylase is produced, to preserve resources and energy for germination, consequently very little starch is broken down, there is why the iodine remains blue black colour and no change of colour was observed.


Furthermore, the control treatments for each stage did not show any amylase activity. This was because the enzyme was denatured after boiling and was not able to break down starch. This shows that amylase was in fact the active agent responsible for hydrolyzing starch, as if it were not starch would have still been broken down after its boiling.


Identifying maltose as the product of starch hydrolysis by amylase


Maltose was produced as the Benedicts reagent reacted with maltose with the mixture to form a cuprous oxide precipitate( as was expected in the experiment it confirms that the amylase enzyme does catalyse the reaction of starch) from maltose in germinating barley seed. In the 4b) the control tube it only confirms that the amylase is responsible for the production of maltose.


During this practical a number of errors may have been made, which could have to some extent altered the results obtained. These mey include measurement errors, improper grinding of the grains, improper cleaning of the equipment between testing the different stages of seed development leading to contamination and even calculation error.


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Wednesday, February 19, 2020

War

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"War is always a betrayal of the young by the old, of


soldiers by politicians, of idealists by cynics."


~ Chris Hedges (The Oregonian)


Our nation has been fighting a battle of power. Power to have control over a cause that has not been fully explained. This cause is tearing nations apart and questions keep flashing in our heads. What is this cause? Why are we at war with a nation halfway around the world? Why isnt other nations supporting us, the most powerful country in the world? Should I be scared? We are a nation of power and prestige, why should I be scared? However, a tragic and devastating event occurred on September 11, 001. Our nation endured and lived through the deaths of thousands of people. Now, we the people of the United States are angry. Angry and scared that we are going to lose all of the power, prestige, freedom, and happiness that we created and live with. Slowly, the cycle of war begins. The focuses of our nation are stressed on the struggles and hardships that have been tolerated. The faults of our 'enemy nations are now pointed out and we are destroying them. Write my Essay on War


Chris Hedges and his book War Is a Far That Brings Us Meaning have brought new theories and explanations to many issues that have been recently been introduced. One theory in particular that has kept my interest has been on the subject of how a state destroys their culture and then attempts to bring down their enemy. What does this mean? First, a state will silence their own culture, and then the "enemy is dehumanized" and finally, everything is targeted toward "promoting and glorifying the myth, the nation, the cause" (Hedges 6). We have seen this constantly in the exact form and pattern. Since September 11th, the United States has forced their people to become an army against Iraq, Afghanistan, and their partners in 'terrorism. The suicide bombers, rulings done by Saddam Hussein and the deaths of thousands under his rule, and corrupt leaders have been pushed into our minds to force us to sympathize with the weak and unfortunate. It forces us to want to change the bad and take control of the situation. As this is occurring, the enemy is seen as immoral, selfish killers, only looking out for themselves. We seen their state and want them to pay for their wrong doings. We forget that they are humans as well. Finally, patriotism is the new target. "United We Stand", "God Bless America", "We the People", and "We Will Never Forget" are just a few to name the many. People are brought together by tragedy and hard times. In the United States case, the September 11th attacks were a reason to bring everyone together. National symbols now became something that is seen everyday. People were proud to be American. Glued to their televisions at night, watching a concert dedicated to New York where U, Faith Hill, Dave Matthews, and many others sang for the nation. Watching how President George W. Bush would speak to the country every night proclaiming our unity. Our nation because focused on the stars and stripes, not the deaths and torture.


Chris Hedges also explained how ones state is thrown into a "trance". A message is pushed how that the cause is just, the state is right, and everything that the states leaders are doing is good (Hedges 64). In the United States case, people are addicted to what they believe is a just cause. But in reality, "we feel guilty". We are "knocked off balance" in our perfect world (Hedges 74). We dont know what to expect and we become frightened of the reality of what is really happening. The killings make our nation angry and force them to stay glued to their televisions, wanting to see update after update. This is one of the most covered wars our nation has ever witnessed. People can see firsthand what is going on over in Iraq. However, we are only seeing what we want to see. This forces a different understanding of our culture and history. The past is "recreated to fit the myth" (Hedges 80). The myth in the case of the recent war with Iraq is the fact that we, the people of the United States of America, are doing a good deed in trying to recreate a government for Iraq.


Peace becomes "possible only when (the) culture no longer serves a cause or a myth but the most precious elusive of all human narratives truth" (Hedges 8). Will we ever know the truth?


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